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As with the rest of the Formula 1 community, the team is shocked and saddened by the invasion of Ukraine and wishes for a swift and peaceful end to the conflict,” Haas said in a statement Saturday.
The 23-year-old Mazepin did not score a point in his debut F1 season last year alongside Mick Schumacher, the son of seven-time F1 champion Michael Schumacher.
Last week, Haas removed Uralkali sponsorship and ran plain white cars on the final day of pre-season testing in Barcelona, instead of the typically red, white and blue with a livery that resembles the Russian flag.
Pre-season testing continues in Bahrain next week before the season-opening race there on March 20.
The Haas Formula One team has terminated its contract with Russian driver Nikita Mazepin and title sponsor Uralkali.
The decision was taken in the wake of Russia's ongoing invasion of Ukraine and means no Russians are signed up to drive in F1 ahead of the new season.
"Haas F1 Team has elected to terminate, with immediate effect, the title partnership of Uralkali, and the driver contract of Nikita Mazepin," the team said in a statement. "As with the rest of the Formula One community, the team is shocked and saddened by the invasion of Ukraine and wishes for a swift and peaceful end to the conflict."
The sport's governing body, the FIA, had kept open the possibility of when it announced this week that Russian and Belarusian drivers would be allowed to continue to compete in international motorsport as long as they raced under a neutral flag and agreed not to express any support of Russia's invasion of Ukraine.
However, Haas announced Saturday that the deal with Mazepin and Uralkali had been terminated, with the team saying news on a replacement driver would follow next week.
An hour after the news broke, Mazepin released a statement via social media.